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Grant Bio EF600M Asymptote


Liquid nitrogen free and cryogen free controlled rate freezer for the cryopreservation of a wide range of material including: embryos, stem cells, mammalian cells, spermatozoa, antibodies, tissue sections and rodent organs. The EF600M brings accuracy, precision and reproducibility to biological cryopreservation.

Unlike conventional liquid Nitrogen based controlled rate cooling equipment used in IVF laboratories, the EF600M poses no contamination risk and can be used in cleanrooms and barrier facilities. The EF600M fits neatly and quietly on a bench-top and its performance in terms of cell viability after freezing is comparable or better than standard liquid nitrogen freezers. As alcohol is not used, there is also no potential fire risk. The EF600M will cool down to – 100ºC with straws.

The cooling rate of the EF600M is precisely controlled, ensuring accuracy and reproducibility throughout the freezing profile especially for the important nucleation/seeding phase. This ensures optimal recovery of cells upon thawing. Operation is simple and can be carried out with or without a PC; data can be logged via PC software and cooling profiles are directly displayed. Different cooling profiles are available from a drop down menu and customised profiles can be written. Published trials have demonstrated successful freezing and recovery of embryos, sperm and embryonic stem cells.

Main Applications

The EF600M is highly versatile and can be used for the cryopreservation of a wide range of samples in cryovials, straws, bags, microplates and Matrix-96-well block plates including:

  Human and veterinary IVF
  Transgenic embryos
  Stem cells
  Clinical and research samples, e.g. lymphocytes and tissue cells lines in conventional cryovials
  Various mammalian cells including cardiomyocytes, adipose, liver and muscle
  Cord blood derived stem cells in typical cryocyte bags
  Adherent cells and stem cells in microplates plates
  Cell suspensions in numbered/barcoded arrays for archiving and storage
  Robotic integration – the EF600M has also successfully been integrated into robotic systems, supporting biorepository and biobank applications


Key Features

  Accurate and reproducible control of cooling rates and sample temperatures
  Easy to use and samples can be nucleated/seeded in-situ
  Linear and non-linear cooling profiles
  Low running costs: estimated at 1% of liquid nitrogen controlled rate freezing
  Temperature remains at -100ºC at the end of cycle for straw applications until freezer is switched off
  Uninterruptible Power Supply (UPS): complete cycle run if power fails (supplied as an optional accessory)
  CE marked
  Servicing and calibration available
  3 year warranty


Data Sheet



Please note - Product designs and specifications are subject to change without notice. The user is responsible for determining the suitability of this product.


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